Immunocytochemistry analysis of Cas9 in HEK293 cells. Samples were stained with Cas9 polyclonal antibody (Product # PA5-143598) in red. Cells were transfected with a construct including the C-terminal 250 amino acids ofS. aureusCAS9 fused to GFP. Transfected cells express the green fusion protein and bind the antibody in red, producing a yellow signal. Nuclear DNA in transfected and non-transfected cells is revealed with the blue DNA stain DAPI.
Supplier Page from Thermo Fisher Scientific for Cas9 Antibody